PAGE 1 000 B000000 11/30/2017 000 C000000 0001293613 000 D000000 N 000 E000000 NF 000 F000000 Y 000 G000000 N 000 H000000 N 000 I000000 6.1 000 J000000 A 001 A000000 KAYNE ANDERSON MLP INVESTMENT COMPANY 001 B000000 811-21593 001 C000000 8776573863 002 A000000 811 MAIN STREET, 14TH FLOOR 002 B000000 HOUSTON 002 C000000 TX 002 D010000 77002 003 000000 N 004 000000 N 005 000000 N 006 000000 N 007 A000000 N 007 B000000 0 007 C010100 1 007 C010200 2 007 C010300 3 007 C010400 4 007 C010500 5 007 C010600 6 007 C010700 7 007 C010800 8 007 C010900 9 007 C011000 10 008 A000001 KA FUND ADVISORS, LLC 008 B000001 A 008 C000001 801-67089 008 D010001 HOUSTON 008 D020001 TX 008 D030001 77002 010 A000001 ULTIMUS FUND SOLUTIONS, LLC 010 B000001 84-58290 010 C010001 CINCINNATI 010 C020001 OH 010 C030001 45246 011 A000001 CITIGROUP GLOBAL MARKETS INC. 011 B000001 8-8177 011 C010001 NEW YORK 011 C020001 NY 011 C030001 10022 011 A000002 UBS SECURITIES, LLC 011 B000002 8-22651 011 C010002 NEW YORK PAGE 2 011 C020002 NY 011 C030002 10019 011 A000003 MERRILL LYNCH, PIERCE, FENNER & SMITH INC. 011 B000003 8-7221 011 C010003 NEW YORK 011 C020003 NY 011 C030003 10080 011 A000004 MORGAN STANLEY & CO. LLC 011 B000004 8-15869 011 C010004 NEW YORK 011 C020004 NY 011 C030004 10036 011 A000005 WELLS FARGO SECURITIES, LLC 011 B000005 8-65876 011 C010005 CHARLOTTE 011 C020005 NC 011 C030005 28202 011 A000006 ROBERT W. BAIRD & CO. INCORPORATED 011 B000006 8-49700 011 C010006 MILWAUKEE 011 C020006 WI 011 C030006 53202 011 A000007 RBC CAPITAL MARKETS LLC 011 B000007 8-45411 011 C010007 NEW YORK 011 C020007 NY 011 C030007 10281 011 A000008 STIFEL NICOLAUS & COMPANY, INCORPORATED 011 B000008 8-1447 011 C010008 ST LOUIS 011 C020008 MO 011 C030008 63102 011 A000009 BARCLAYS CAPITAL INC. 011 B000009 8-51376 011 C010009 NEW YORK 011 C020009 NY 011 C030009 10019 011 A000010 DEUTSCHE BANK SECURITIES INC. 011 B000010 8-74042 011 C010010 NEW YORK 011 C020010 NY 011 C030010 10005 011 A000011 OPPENHEIMER & CO. INC. 011 B000011 8-04077 011 C010011 NEW YORK 011 C020011 NY 011 C030011 10004 011 A000012 LADENBURG THALMANN & CO. INC. 011 B000012 8-17230 011 C010012 NEW YORK 011 C020012 NY PAGE 3 011 C030012 10022 012 A000001 AMERICAN STOCK TRANSFER & TRUST CO., LLC 012 B000001 84-00416 012 C010001 BROOKLYN 012 C020001 NY 012 C030001 11219 013 A000001 PRICEWATERHOUSECOOPERS LLP 013 B010001 LOS ANGELES 013 B020001 CA 013 B030001 90071 014 A000001 KA ASSOCIATES, INC. 014 B000001 8-45640 015 A000001 JPMORGAN CHASE BANK, N.A. 015 B000001 C 015 C010001 DALLAS 015 C020001 TX 015 C030001 75254 015 E010001 X 018 000000 Y 019 A000000 Y 019 B000000 4 019 C000000 KAYNEANDER 020 A000001 CITIGROUP GLOBAL MARKETS, INC. 020 B000001 11-2418191 020 C000001 83 020 A000002 JONES TRADING INSTITUTIONAL SERVICES, LLC 020 B000002 51-0484896 020 C000002 51 020 A000003 WELLS FARGO SECURITIES, LLC 020 B000003 56-2326000 020 C000003 68 020 A000004 UBS SECURITIES, INC. 020 B000004 13-3873456 020 C000004 80 020 A000005 GOLDMAN SACHS & CO. 020 B000005 13-5108880 020 C000005 27 020 A000006 INSTINET LLC 020 B000006 13-2625132 020 C000006 52 020 A000007 USCA SECURITIES LLC 020 B000007 22-3696585 020 C000007 32 020 A000008 LIQUIDNET, INC. 020 B000008 13-2655998 020 C000008 42 020 A000009 BARCLAYS CAPITAL, INC. 020 B000009 06-1031656 020 C000009 39 020 A000010 JEFFRIES & CO., INC.S LLC 020 B000010 95-2622900 PAGE 4 020 C000010 23 021 000000 643 022 A000001 UBS SECURITIES, INC. 022 B000001 13-3873456 022 C000001 18540 022 D000001 0 022 A000002 CREDIT SUISSE FIRST BOSTON (USA) LLC 022 B000002 05-0546650 022 C000002 21684 022 D000002 0 022 A000003 BARCLAYS CAPITAL, INC. 022 B000003 06-1031656 022 C000003 15583 022 D000003 0 022 A000004 MORGAN STANLEY & CO., LLC. 022 B000004 13-2655998 022 C000004 15038 022 D000004 0 022 A000005 CITIGROUP GLOBAL MARKETS, INC. 022 B000005 11-2418191 022 C000005 50096 022 D000005 0 022 C000006 0 022 D000006 0 022 C000007 0 022 D000007 0 022 C000008 0 022 D000008 0 022 C000009 0 022 D000009 0 022 C000010 0 022 D000010 0 023 C000000 120941 023 D000000 0 024 000000 N 025 D000001 0 025 D000002 0 025 D000003 0 025 D000004 0 025 D000005 0 025 D000006 0 025 D000007 0 025 D000008 0 026 A000000 N 026 B000000 Y 026 C000000 N 026 D000000 Y 026 E000000 N 026 F000000 N 026 G010000 N 026 G020000 N PAGE 5 026 H000000 N 027 000000 N 028 A010000 0 028 A020000 0 028 A030000 0 028 A040000 0 028 B010000 0 028 B020000 0 028 B030000 0 028 B040000 0 028 C010000 0 028 C020000 0 028 C030000 0 028 C040000 0 028 D010000 0 028 D020000 0 028 D030000 0 028 D040000 0 028 E010000 0 028 E020000 0 028 E030000 0 028 E040000 0 028 F010000 0 028 F020000 0 028 F030000 0 028 F040000 0 028 G010000 0 028 G020000 0 028 G030000 0 028 G040000 0 028 H000000 0 045 000000 Y 046 000000 N 047 000000 Y 048 000000 0.000 048 A010000 4500000 048 A020000 1.375 048 B010000 5000000 048 B020000 1.250 048 C010000 5000000 048 C020000 1.125 048 D010000 0 048 D020000 0.000 048 E010000 0 048 E020000 0.000 048 F010000 0 048 F020000 0.000 048 G010000 0 048 G020000 0.000 048 H010000 0 048 H020000 0.000 PAGE 6 048 I010000 0 048 I020000 0.000 048 J010000 0 048 J020000 0.000 048 K010000 14500000 048 K020000 1.000 049 000000 N 050 000000 N 051 000000 N 052 000000 N 053 A000000 Y 053 B000000 Y 053 C000000 N 054 A000000 Y 054 B000000 Y 054 C000000 Y 054 D000000 N 054 E000000 N 054 F000000 N 054 G000000 Y 054 H000000 Y 054 I000000 N 054 J000000 Y 054 K000000 N 054 L000000 N 054 M000000 Y 054 N000000 N 054 O000000 N 055 A000000 N 055 B000000 Y 056 000000 Y 057 000000 N 058 A000000 N 059 000000 Y 060 A000000 N 060 B000000 N 061 000000 0 062 A000000 N 062 B000000 0.0 062 C000000 0.0 062 D000000 0.0 062 E000000 0.0 062 F000000 0.0 062 G000000 0.0 062 H000000 0.0 062 I000000 0.0 062 J000000 0.0 062 K000000 0.0 062 L000000 0.0 062 M000000 0.0 062 N000000 0.0 PAGE 7 062 O000000 0.0 062 P000000 0.0 062 Q000000 0.0 062 R000000 0.0 063 A000000 0 063 B000000 0.0 066 A000000 Y 066 B000000 N 066 C000000 N 066 D000000 N 066 E000000 N 066 F000000 N 066 G000000 Y 067 000000 N 068 A000000 N 068 B000000 N 069 000000 N 070 A010000 Y 070 A020000 N 070 B010000 Y 070 B020000 Y 070 C010000 Y 070 C020000 N 070 D010000 Y 070 D020000 N 070 E010000 Y 070 E020000 N 070 F010000 Y 070 F020000 N 070 G010000 Y 070 G020000 N 070 H010000 Y 070 H020000 N 070 I010000 Y 070 I020000 N 070 J010000 Y 070 J020000 Y 070 K010000 Y 070 K020000 N 070 L010000 Y 070 L020000 N 070 M010000 N 070 M020000 N 070 N010000 Y 070 N020000 N 070 O010000 Y 070 O020000 Y 070 P010000 N 070 P020000 N 070 Q010000 Y 070 Q020000 N PAGE 8 070 R010000 Y 070 R020000 N 071 A000000 674202 071 B000000 804528 071 C000000 3830246 071 D000000 18 072 A000000 12 072 B000000 83 072 C000000 20767 072 D000000 0 072 E000000 0 072 F000000 52324 072 G000000 1210 072 H000000 0 072 I000000 29 072 J000000 203 072 K000000 0 072 L000000 315 072 M000000 448 072 N000000 138 072 O000000 -25344 072 P000000 29576 072 Q000000 0 072 R000000 472 072 S000000 94 072 T000000 0 072 U000000 0 072 V000000 0 072 W000000 12763 072 X000000 72228 072 Y000000 0 072 Z000000 -51378 072AA000000 384267 072BB000000 178243 072CC010000 0 072CC020000 313771 072DD010000 60863 072DD020000 0 072EE000000 0 073 A010000 0.5300 073 A020000 0.0000 073 B000000 0.0000 073 C000000 1.3700 074 A000000 2000 074 B000000 0 074 C000000 75305 074 D000000 0 074 E000000 132520 074 F000000 3183260 074 G000000 0 074 H000000 0 PAGE 9 074 I000000 0 074 J000000 4336 074 K000000 0 074 L000000 465 074 M000000 1447 074 N000000 3399333 074 O000000 4283 074 P000000 11896 074 Q000000 747000 074 R010000 0 074 R020000 0 074 R030000 0 074 R040000 517981 074 S000000 292000 074 T000000 1826173 074 U010000 114877 074 U020000 0 074 V010000 15.90 074 V020000 0.00 074 W000000 0.0000 074 X000000 54 074 Y000000 0 075 A000000 0 075 B000000 2128965 076 000000 15.32 077 A000000 Y 077 B000000 Y 077 Q020000 Y 078 000000 N 080 A000000 FEDERAL INSURANCE COMPANY 080 B000000 NONE 080 C000000 2500 081 A000000 N 081 B000000 0 082 A000000 N 082 B000000 0 083 A000000 N 083 B000000 0 084 A000000 N 084 B000000 0 085 A000000 Y 085 B000000 N 086 A010000 1190 086 A020000 21335 086 B010000 0 086 B020000 0 086 C010000 0 086 C020000 0 086 D010000 320 086 D020000 8000 086 E010000 0 PAGE 10 086 E020000 0 086 F010000 20000 086 F020000 20000 087 A010000 KAYNE ANDERSON MLP INVESTMENT COMPANY 087 A020000 486606106 087 A030000 KYN 088 A000000 Y 088 B000000 N 088 C000000 Y 088 D000000 Y SIGNATURE TERRY A. HART TITLE CFO